Blogs about: Fluorescent Protein

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Photoactivated Transcription Revisted

andrewhires wrote 4 days ago: Looks like there has been some new results in the field of photoactivated transcription.  Unlike the … more →

Tags: imaging, Neuronal Control, photoactivation, Doxycyclin, tet-on

Infrared fluorescent proteins5 comments

andrewhires wrote 2 months ago: Hunting for new fluorescent proteins in the coral reefs of the Caribbean and Australia is a task tha … more →

Tags: GFP, imaging, in vitro, in vivo

Background : Perceval, the ATP:ADP sensor1 comment

andrewhires wrote 4 months ago: Recently, Brain Windows mentioned the report A genetically encoded fluorescent reporter of ATP:ADP … more →

Tags: fret, imaging, ATP

Symposium : A Revolution in Fluorescence Imaging5 comments

andrewhires wrote 5 months ago: This coming Tuesday and Wednesday (Feb 17th & 18th) at UCSD, there will be a symposium honorin … more →

Tags: Brain imaging, Calcium, fret, GFP, imaging, in vitro, in vivo, Meeting, geci

BrainStorm 1 : The Calcium Memory Sensor

andrewhires wrote 6 months ago: As mentioned in the previous post, this is the first installment of BrainStorm, a section of ideas I … more →

Tags: Brain imaging, brainstorm, Calcium, imaging, in vivo, Multiphoton

Fluorescent Proteins in Scholarpedia

andrewhires wrote 7 months ago: I just discovered that the scholarpedia article on fluorescent proteins was published back in July. … more →

Preview : Structure of G-CaMP21 comment

andrewhires wrote 7 months ago: A high-resolution crystal structure of the genetically-encoded calcium indicator G-CaMP2 would aid i … more →

Tags: Calcium, GFP, imaging, in vitro, society for neuroscience, Structure, Fluorescent, geci, preview

Some interesting posters @ SfN2 comments

andrewhires wrote 8 months ago: Here’s a few posters that caught my eye at SfN.  Click the meeting planner for the full abstra … more →

Tags: Calcium, GFP, imaging, in vitro, in vivo, Multiphoton, society for neuroscience, Neuroscience, SFN

Raw Data : Vesicular Release from Astrocytes, SynaptopHluorange

andrewhires wrote 8 months ago: When I was working on my Ph.D. thesis, I was trying to find some biological question to definitively … more →

Tags: Calcium, Glutamate, imaging, in vitro, Photobleaching, visualization, Fluorescence, pHluorin

A molecular clock1 comment

davidkirkpatrick wrote 8 months ago: This is a cool story from Technology Review. From the link: A Fast, Programmable Molecular Clock Th … more →

Tags: Science, technology, technology review, university of california, san diego, e. coli, Emily Singer, molecular clock, genetic clock

2008 Nobel Prize in Chemistry to GFP6 comments

andrewhires wrote 9 months ago: This morning, the Nobel committee recognized the work of Osamu Shimomura, Martin Chalfie and Roger T … more →

Tags: imaging, Calcium, Glutamate, visualization, GFP, Nobel Prize

The great GECI shootout

andrewhires wrote 12 months ago: Dierk Reiff’s lab has done another head-to-head in vivo showdown between various GECIs and a s … more →

Tags: Calcium, fret, imaging, in vitro, in vivo

Sensing salty currents with Mermaids3 comments

andrewhires wrote 1 year ago: A new genetically-encoded voltage sensor paper is out from a friend and former mentor of mine, Atsus … more →

Tags: fret, imaging, in vitro, voltage, voltage sensor

Voltage sensitive imaging powering up7 comments

andrewhires wrote 1 year ago: I’m starting to come around on voltage imaging. I haven’t been a fan of it for a number … more →

Tags: fret, imaging, in vitro, in vivo, Small-molecule, voltage, dpa, Fluorescence, Quenching

SLICK labeling and new FPs

andrewhires wrote 1 year ago: There is a nice writeup of the single-neuron labeling with inducible Cre-mediated knockout (SLICK) p … more →

Tags: imaging, in vitro, in vivo, Photobleaching, knockout

Giving synapses a 'born on' label

andrewhires wrote 1 year ago: Memories are thought to be encoded by the patterns of synaptic connections in the brain. Learning ca … more →

Tags: imaging, in vitro, in vivo, fluorescent label, timestamp

Journal Club : GFP Reconstitution Across Synaptic Partners (GRASP)

andrewhires wrote 1 year ago: This week, I’m guest presenting a recent Neuron paper over at the Nature Network’s neuro … more →

Tags: Blogroll, Blog, journal club, Nature, synapse

Optical imaging of neuronal glutamate release and spillover with GluSnFR3 comments

andrewhires wrote 1 year ago: This post is difficult to craft. I’ve been struggling with whether to write an epic post descr … more →

Tags: fret, Glutamate, imaging, in vitro, Neuron, spillover

3D and Multicolor Superresolution Imaging1 comment

andrewhires wrote 1 year ago: Progress in superresolution imaging is still moving very quickly. Here are two more great papers in … more →

Tags: imaging, Photobleaching, Super-Resolution, Superresolution


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