Blogs about: Lab Activities
Gel electrophoresis of PCRjan1508-5p3p
Note Taker wrote 1 year ago: 3% agarose, 0.5x TBE (25oml), 16 microlitres of sample ladder: low ladder, 8 microlitre Gel 1: A1-12 … more →
PCR Jan1508-5p3p
Note Taker wrote 1 year ago: PCR to test L seedlings (and screen* new primers) – PCR Jan1508-5p3p Primers: 1720/1721, 1724/25, 17 … more →
Gel electrophoresis of PCRjan1008-8p - 1
Note Taker wrote 1 year ago: Gel 1-Jan 11th Top row: A1-12, ladder, B1-12 Middle row: C1-12, ladder, D1-12 Ladder: Low ladder 8mi … more →
Gel electrophoresis of PCRjan0408-2p
Note Taker wrote 1 year ago: Poured gel Jan 7th. 250ml 3% agarose, 0.5 x TBE Loaded Jan 8th, Run at ~28 mA (slow run) Loading ord … more →
PCR Jan0308-2p
Note Taker wrote 1 year ago: Primers: 1180/1181 and 1150/1151 DNA: L1-L13′ (26 samples) A1-C2, C12: 1180/1181 D1-F2, F12: P … more →
PCR dec0607-4p
Note Taker wrote 2 years ago: Primers: 118/119 (Val’s box), 27/28 (new dil.), 35/36 (new dil. previously used), 1180/1181 Ta … more →
Gel electrophoresis of PCRdec0407-1p
Note Taker wrote 2 years ago: 3% agarose gel (7.5g) poured Dec4th, 250ml 0.5x TBE Starting current: 46 mA Load samples @ 8:55am To … more →
PCR dec0407-1p
Note Taker wrote 2 years ago: Primer 118/119 Polymerase: Biolase (0.4 µl) Template: L1-L13, JA 1/21 (as +’ve ctrl, old and n … more →
Gel electrophoresis of PCRnov3007-2p
Note Taker wrote 2 years ago: Agarose 3%, 250ml, 0.5xTBE (Fresh soln for tank) Starting Voltage: ~40 mA switched to 30 mA after 22 … more →
Gel electrophoresis of PCRnov2707-1p
Note Taker wrote 2 years ago: 0.5x TBE (MB prep) Start Time: ~ 10:00 am ~15 microlitres loaded, 8 microlitres ladder Results: only … more →
PCR - nov2707-1p
Note Taker wrote 2 years ago: Primer: 35/36 DNA: L1, L1’ L6, L6’, L12, L12’, L13, L13’ Conditions: New buffer, new MgCl2, new dNTP … more →
Gel electrophoresis of PCR
Note Taker wrote 2 years ago: PCR date: Oct 11, 07 27/28: L1-L7’, L13, L13’ (AB) 82/83: all (CD, H) 35/36: L6, L6’, L12, L12’, L13 … more →
PCR 11Oct07
Note Taker wrote 2 years ago: 27/28: L1-L7’, L13, L13’ (AB) 82/83: all (CD, H) 35/36: L6, L6’, L12, L12’, L13, L13’ (E) 43/44: L6, … more →
Verification
Note Taker wrote 2 years ago: Recommended Primers for Verification Primer ID Positive Ctrl Recommended program: SSR 51 Recommended … more →
Gel electrophoresis etc
Note Taker wrote 2 years ago: Electrophoresis of PCR set up by J. Included samples from test PCR: D1-9 Gel results: J’s PCR: … more →
PCR results
Note Taker wrote 2 years ago: Gel done today for PCR from Feb 13th, shows that BIOTAQ works better than the TAQ that was at RT so … more →
PCR to check Taq
Note Taker wrote 2 years ago: DNA Templates: First D-squared DNA (3 samples: L1, L2, L3) Second D-squared DNA (3 samples: BO33, 3 … more →
DNA extraction - UL samples
Note Taker wrote 2 years ago: D-squared kit extraction: 26 unlabeled samples from greenhouse, SSM’s seedlings - Jan 29-31, … more →
BN 25-72, BO 1-8 etc
Note Taker wrote 3 years ago: BN 25-48 Extracted Nov 20th (Kobayashi method) BN 49-72 Extracted Nov 22nd (Excluding 53-58 which wa … more →
