Tags » Experimental Design

Imaging SmartFlares +/- DMOG (Live)

This is a final repeat of the basic SmartFlare experiment. HeLa cells are loaded with the three variants of SmartFlares and imaged 18h later. 104 more words


Imaging SmartFlares +/- DMOG (Live)

The purpose of these experiments is to be able to visualise the SmartFlares (SFs) in the cell after having followed the loading protocol provided by the manufacturers. 242 more words


The Real Scientific Method

I might have to edit this or add another post once I am able to describe the specific study that inspired this post. However, were the problem mainly about that study, I wouldn’t be posting anything. 1,388 more words

Research Methods

We Can’t All Be Math Nerds & Science Geeks

This is the title of a Outlook piece in the March 29th edition of the Washington Post. by Fareed Zakaria.  His article excoriates our obsession with STEM (Science, Technology, Engineering, and Mathematics) education and argues that it will make it harder for America to innovate.  526 more words

Human Memory: Theory And Data

Cancer: Hope for sale? (Panorama)

Broadcaster: BBC1

Year: 2013

Genre: Documentary

URL: http://bobnational.net/record/265685

A 30 minute episode of the current affairs Panorama series, focusing on the controversial “antineoplastins” cancer therapy promoted by Texan doctor Stanislaw Burzynski. 60 more words


How much is enough? A new technique for quantifying precision of community surveys

Being an ecologist is all about the trade-off between effort, and time and money. Given infinite amounts of both, we would undoubtedly sample the heck out of nature. 2,010 more words


Use of dimethyloxalylglycine (DMOG)

The SmartFlare that we have chosen for this project is specific to vascular endothelial growth factor (VEGF) mRNA.

VEGF was selected as the target because it’s transcript becomes increased in a cell as part of a characterized response to hypoxia with the aid of hypoxia inducible factor-1 (HIF-1). 163 more words