Tags » Protocols

Trouble-shooting genome size estimates with flow cytometry

According to Johnston, to get a good estimate we should make sure to properly dounce the head nuclei and run samples as slowly as possible. 229 more words

Genome Size

Generating Y-replacement lines to study effects of heterochromatin size

In this post, I briefly outline the genetic crosses used to achieve Y-replacement lines and what biological and genomic assays we will use them for later. 549 more words

Genome Size

Protocol flow cytometry

Johnston Spencer shared his flow cytometry protocol with us (methods Hare & Johnston). His advice in his email:

My methods are given in the greatest detail in the attached, except we now use 25ug /ml, not 50. 374 more words


JProwler - A simple Wireless Network Simulator in Java

When I was a visiting researcher at the Institute of Software Integrated Systems at Vanderbuilt University in 2005, I learnt about a Wireless Network Simulator named JProwler. 186 more words


Shrinks who designed CIA torture admit to torturing -- but say it wasn't torture!

Bruce Jessen — Jim Mitchell

The acknowledgement by two former Air Force psychologists that they used waterboarding and other harsh tactics against detainees in the war on terror is a striking development…

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Key Management Interoperability Protocol (KMIP)

Key Management Interoperability Protocol (KMIP): Centralized encryption key management based on industry standards

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What is the digital difference in Networks vs. Protocols?

Like many words in the English language, “network” and “protocol” have different meanings, depending on the context. For example, your interpersonal network is far different from a railway network.

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