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Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches

Bhaskar Chanda Stem Cell Miki et al. develop synthetic miRNA switches for isolating cell populations that are otherwise difficult to purify. Several miRNA-responsive switches precisely and efficiently isolate human pluripotent stem cell (hPSC)-derived cardiomyocytes, and miRNA switches can be programmed for purification of various cell types including hPSC-derived endothelial cells, hepatocytes, and insulin-producing cells. 12 more words

Scientist

Combined Single-Cell Functional and Gene Expression Analysis Resolves Heterogeneity within Stem Cell Populations

Bhaskar Chanda Stem Cell Wilson et al. combine single-cell functional assays with flow cytometric index sorting and single-cell gene expression assays to reveal gene expression programs of HSCs with durable self-renewal potential in transplantation assays. 31 more words

Scientist

An RNA-Containing Negative Feedback Loop Regulates p53-Mediated Apoptosis in Embryonic Stem Cells

Bhaskar Chanda Stem Cell Li et al. show that an ESC-enriched regulatory RNA, Apela, positively regulates p53-mediated DNA damage-induced apoptosis (DIA) of ESCs. Apela, hnRNPL, and p53 form a tri-element negative feedback loop that may help ESCs navigate the balance between maintaining inactive p53 under baseline conditions and eliciting DIA upon DNA damage. 12 more words

Stem Cell

Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity in Pluripotency and Reprogramming

Bhaskar Chanda Stem Cell Using Sox2-centered interactome analysis, Ding et al. identify Tex10 as an evolutionarily conserved key pluripotency factor that plays a critical role in ESC self-renewal and pluripotency, early embryo development, and somatic cell reprogramming via epigenetic regulation of super-enhancer activity. 12 more words

Stem Cell

Filia Is an ESC-Specific Regulator of DNA Damage Response and Safeguards Genomic Stability

Bhaskar Chanda Stem Cell Zhao et al. identify Filia as an ESC-specific regulator of genomic stability that is induced by genotoxic stress. Functional characterization highlights dynamic subcellular translocation and roles in governing multiple DNA damage response pathways, including stimulation of PARP1 enzymatic activity. 12 more words

Stem Cell

Erosion of X Chromosome Inactivation in Human Pluripotent Cells Initiates with Coating and Depends on a Specific Heterochromatin Landscape

Bhaskar Chanda Stem Cell Rougeulle and colleagues show that XCI erosion in human pluripotent cells is related to a pluripotency-specific epigenomic landscape on the inactive X chromosome, and that coating by the XACT lncRNA and gene reactivation precedes loss of XIST expression during this process. 12 more words

Scientist

β-Catenin Regulates Primitive Streak Induction through Collaborative Interactions with SMAD2/SMAD3 and OCT4

Bhaskar Chanda Stem Cell In this article, Funa, Schachter, and colleagues et al. provide mechanistic insights into how Wnt signaling controls early cell lineage decisions. β-catenin occupies regulatory regions in numerous PS and neural crest genes, and direct interactions between β-catenin and SMAD2/SMAD3 in proximity to OCT4 binding are required to specifically activate PS genes. 12 more words

Scientist