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Erosion of X Chromosome Inactivation in Human Pluripotent Cells Initiates with Coating and Depends on a Specific Heterochromatin Landscape

Bhaskar Chanda Stem Cell Rougeulle and colleagues show that XCI erosion in human pluripotent cells is related to a pluripotency-specific epigenomic landscape on the inactive X chromosome, and that coating by the XACT lncRNA and gene reactivation precedes loss of XIST expression during this process. 12 more words

Scientist

β-Catenin Regulates Primitive Streak Induction through Collaborative Interactions with SMAD2/SMAD3 and OCT4

Bhaskar Chanda Stem Cell In this article, Funa, Schachter, and colleagues et al. provide mechanistic insights into how Wnt signaling controls early cell lineage decisions. β-catenin occupies regulatory regions in numerous PS and neural crest genes, and direct interactions between β-catenin and SMAD2/SMAD3 in proximity to OCT4 binding are required to specifically activate PS genes. 12 more words

Scientist

Organ donation and infants: One family’s brave story about love, loss and helping others

His liver was a match for a little boy waiting for a donor, his kidney took an adult off of dialysis and his heart is now beating inside of a little girl whose life he saved. 1,320 more words

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How one woman’s organs saved six lives

WATCH ABOVE: How one family helped save six lives by saying yes to organ donation. Crystal Goomansingh reports.

TORONTO – Her kidneys gave a second chance to a man and a young girl, while her pancreas saved a woman living with diabetes. 702 more words

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Opposing Roles for the lncRNA and Its Genomic Locus in Regulating Gene Activation during Embryonic Stem Cell Differentiation

Bhaskar Chanda Stem Cell Dissection of the precise contribution of RNA transcripts versus genomic DNA sequences is important for functional assessment of long noncoding RNAs. Here, Shen and colleagues demonstrate discrete and opposing roles for the lncRNA transcript Haunt and its genomic locus in regulating the HOXA gene cluster during embryonic stem cell differentiation. 12 more words

Scientist

Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation

Bhaskar Chanda Stem Cell Takebe et al. report a generalized method for organ bud formation from diverse tissues, including kidney, pancreas, intestine, heart, lung, and brain, using heterotypic cell mixtures including mesenchymal stem cells to guide cell condensation. 27 more words

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ERRs Mediate a Metabolic Switch Required for Somatic Cell Reprogramming to Pluripotency

Bhaskar Chanda Stem Cell Kida et al. show that upregulation of ERRα or ERRγ is required for a metabolic switch involving OXPHOS that is essential for iPSC generation and that early upregulation of ERRs marks cells that are destined to go on to be reprogrammed. 14 more words

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