Tags » UHN

Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States

Bhaskar Chanda Stem Cell Collier et al. use profiling to identify cell surface proteins that are specific for naive versus primed human pluripotent cells and then use them to isolate and characterize live naive cells arising during primed-to-naive resetting. 13 more words

Scientist

Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution

Bhaskar Chanda Stem Cell CLEVER-seq is a single-cell, single-base resolution whole-genome 5fC-sequencing technology. CLEVER-seq reveals intrinsic 5fC heterogeneity in mouse early embryos, EpiSCs, and ESCs and dissects its highly patterned genomic distributions. 32 more words

Scientist

Amitosis of Polyploid Cells Regenerates Functional Stem Cells in the Intestine

Bhaskar Chanda Stem Cell Lucchetta and Ohlstein describe an unexpected mechanism of stem cell replacement in the Drosophila intestine. They found that in some situations where many stem cells are lost, they can be replaced through a process of ploidy reduction, or amitosis, of differentiated polyploid cells to regenerate functional stem cells. 13 more words

Scientist

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration

Bhaskar Chanda Stem Cell Ambrosi, Schulz, and colleagues define a stem cell-like population that gives rise to osteogenic progeny and promotes hematopoietic reconstitution. Aging and high-fat diet reprogram the mesenchymal lineage to preferentially give rise to adipogenic cells that impair reconstitution and bone fracture healing. 22 more words

Scientist

Mex3a Marks a Slowly Dividing Subpopulation of Lgr5+ Intestinal Stem Cells

Bhaskar Chanda Stem Cell Lgr5+ intestinal stem cells are considered to be a homogeneous and rapidly proliferating population. Barriga et al. show that the RNA binding protein Mex3a defines a subset of slowly proliferating Lgr5+ cells that contribute to all intestinal lineages with slow kinetics, are resistant to chemotherapy, and support intestinal regeneration. 13 more words

Scientist

PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation

Bhaskar Chanda Stem Cell Cruz-Molina et al. demonstrate that pluripotent-associated poised enhancers are necessary for the induction of anterior neural genes. Poised enhancers physically interact with their target genes in embryonic stem cells in a PRC2-dependent manner. 29 more words

Scientist

Targeting BMI1 Cancer Stem Cells Overcomes Chemoresistance and Inhibits Metastases in Squamous Cell Carcinoma

Bhaskar Chanda Stem Cell Chen et al. show that BMI1+ CSCs drive invasive growth and cervical lymph node metastasis in squamous cell carcinoma. BMI1+ CSCs have increased AP-1 activity and are chemotherapy resistant, and combination therapy that targets BMI1+ CSCs and the tumor bulk yields better outcomes and effectively eliminates metastasis. 12 more words

Scientist