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Generation of Cynomolgus Monkey Chimeric Fetuses using Embryonic Stem Cells

Bhaskar Chanda Stem Cell Chen et al. showed that it is feasible to generate chimeric monkeys with contribution in all three germ layers using culture-induced naive-like state embryonic stem cells, opening up new avenues for the use of non-human primate models to study both pluripotency and human disease. 12 more words


Transcriptional Mechanisms of Proneural Factors and REST in Regulating Neuronal Reprogramming of Astrocytes

Bhaskar Chanda Stem Cell Masserdotti et al. analyzed early transcriptional changes mediated by Neurog2 and Ascl1 during direct reprogramming of murine postnatal astrocytes into distinct neuronal subtypes in vitro. 51 more words


Aspp1 Preserves Hematopoietic Stem Cell Pool Integrity and Prevents Malignant Transformation

Bhaskar Chanda Stem Cell Suda and colleagues show that the hematopoietic stem cell (HSC) pool integrity is coordinately controlled by p53 and its proapoptotic activator Aspp1. 41 more words


Sox9 Controls Self-Renewal of Oncogene Targeted Cells and Links Tumor Initiation and Invasion

Bhaskar Chanda Stem Cell Using mouse models of BCC, Larsimont et al. show that Sox9 is required for self-renewal of oncogene-expressing cells and BCC formation. Transcriptional profiling combined with ChIP sequencing uncovered a cancer-specific gene network regulated by Sox9 that promotes stemness, represses differentiation, and induces ECM and cytoskeleton remodeling required for tumor invasion. 13 more words


Functionally Distinct Subsets of Lineage-Biased Multipotent Progenitors Control Blood Production in Normal and Regenerative Conditions

Bhaskar Chanda Stem Cell Pietras et al. show that HSCs produce functionally distinct lineage-biased MPPs that work together to adapt blood production to hematopoietic demands. Two rare subsets of myeloid-biased MPPs are important for maintaining blood homeostasis at steady state and serve as a potent source of myeloid amplification in regenerative conditions. 12 more words


Epigenetic Regulation of Phosphodiesterases 2A and 3A Underlies Compromised β-Adrenergic Signaling in an iPSC Model of Dilated Cardiomyopathy

Bhaskar Chanda Stem Cell In this paper, Wu et al. profiled the β-adrenergic signaling properties in human iPSC-CMs and demonstrated novel epigenetic mechanisms that underlie the compromised β-adrenergic signaling in DCM, a common cause of heart failure and cardiac transplantation. 26 more words


Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches

Bhaskar Chanda Stem Cell Miki et al. develop synthetic miRNA switches for isolating cell populations that are otherwise difficult to purify. Several miRNA-responsive switches precisely and efficiently isolate human pluripotent stem cell (hPSC)-derived cardiomyocytes, and miRNA switches can be programmed for purification of various cell types including hPSC-derived endothelial cells, hepatocytes, and insulin-producing cells. 12 more words

Stem Cell